DNA Logic Multiplexing Using Toehold-Mediated Strand Displacement

Congzhou Chen,Hongyu Wang,Enqiang Zhu,Xiaolong Shi,Jin Xu

doi:10.1109/access.2020.2989444

Abstract

Since the discovery of the DNA Strand Displacement mechanism, researchers have implemented a lot of applications, such as DNA computing, DNA Circuits, Logic gates, and Chemical Reaction network. To achieve those functions, a well-designed system is essential, among which the toehold domains and migration domains play a vital role. In this paper, we designed three basic logic gates based on the toehold mediates DNA strand displacement mechanism, and utilized them to struct a three-layer multiplexer DNA logic circuit. However, the traditional INHIBIT gate annihilated all the inputs strands which obscure the multiplexer in further applications. Therefore, we improved the INHIBIT gate, so the desired input strand can be selected, and the corresponding output strand can be identified. Lastly, we adjusted the multiplexer and realized a cyclic DNA circuit. The simulation results verified the efficiency and reliability of our multiplexer DNA logic circuits. Our method has the ability in architecting complex DNA integrated circuits.

Highlights

DNA as a natural material is an excellent tool for many applications
BASIC LOGIC GATES The primary logic gate, such as AND, OR, INHIBIT can be integrated into more complex circuits
As shown in the AND gate, the output strand has two domains that connected with the substrate

Summary

Introduction

DNA as a natural material is an excellent tool for many applications. It was used as a novel material for nanostructures, as its length and diameter are in nanometer [1]–[4]. INDEX TERMS DNA strand displacement, logic circuits, INHIBIT gate, demultiplexer. In the DNA-based circuits, most gate motifs are useonce only, which are strands consuming. We improved the INHIBIT gate and used it as a demultiplexer so that different input signals were selected and different output signals can be identified.

Results

Conclusion