Abstract
DNA computing plays an important role in nanotechnology due to the unique programmability and parallelism of DNA molecules. As an important tool to realize DNA computation, various logic computing devices have great application potential. The application of DNAzyme makes the achievements in the field of logical computing more diverse. In order to improve the efficiency of the logical units run by DNAzyme, we proposed a strategy to regulate the DNA circuit by the conformational change of the E6-type DNAzyme recognition arms driven by Mg2+. This strategy changes the single mode of DNAzyme signal transmission, extends the functions of E6-type DNAzyme, and saves the time of signal transmission in the molecular scale. To verify the feasibility of this strategy, first, we constructed DNA logic gates (YES, OR, and AND). Second, we cascade different logic gates (YES–YES, YES–AND) to prove the scalability. Finally, a self-catalytic DNA circuit is established. Through the experimental results, we verified that this DNAzyme regulation strategy relatively reduces the cost of logic circuits to some extent and significantly increases the reaction rate, and can also be used to indicate the range of Mg2+ concentrations. This research strategy provides new thinking for logical computing and explores new directions for detection and biosensors.
Highlights
DNA molecules, which follow the principle of complementary base pairing, have the characteristics of programmability and high-density storage, and have gradually become a hot research material
In order to improve the efficiency of the logical units run by DNAzyme, we proposed a strategy to regulate the DNA circuit by the conformational change of the E6type DNAzyme recognition arms driven by Mg2+
Due to its high speci city and catalytic efficiency, enzymes, including restriction endonuclease[18] and DNAzyme,[19] are o en used as DNA strand cutting tools to participate in logic DNA computing,[20] providing technical support in DNA computing, which is commonly used in DNA computing.[21]
Summary
DNA molecules, which follow the principle of complementary base pairing, have the characteristics of programmability and high-density storage, and have gradually become a hot research material. DNA materials are used in combination with DNA nanotechnology, such as strand replacement,[1] DNA tiles,[2] origami,[3] nanoparticle assembly with DNA,[4] etc., has made great achievements in the elds of biochemistry, computing, information and medicine.[5] Based on these technologies, people have designed DNA switches,[6] DNA regenerators,[7] encryption systems,[8] detection devices,[9] nano-robots[10] and other DNA driven devices This is speci c application of a nano-logic circuit,[11] one of the research hotspots due to its great potential in logic calculation,[12] data processing[13] and information sensing,[14] biochemical reaction network,[15] neural networks[16] and catalytic reaction networks[17] and other modules practically. Native polyacrylamide gel electrophoresis and a uorescence assay were used to verify the feasibility and robustness of E6 DNAzyme conformational changes to regulate DNA circuit strategies
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