Abstract
Abstract DNA methylation is catalyzed by DNA methyltransferase (DAM) and plays an important role in many biological processes. The monitoring of DAM activity is of great significance in disease diagnosis. In this paper, an electrochemical DNA biosensor was developed for DAM detection via recognition of DNA tetrahedron and SiO2@Au core/shell nanoparticles-based amplification technique. We immobilize the DNA tetrahedron on the gold electrode surface to construct a well sensing interface. SiO2@Au core/shell nanoparticles was selected to achieve signal amplification for improving the sensitivity. The detection limit of DAM activity by the proposed method was reduced down to 0.0005 U mL−1. The proposed sensing system also showed ideal specificity, repeatability and stability. Moreover, the novel sensor developed could serve as a highly sensitive probe for DAM detection and shows great potentials in designed drug therapy, clinical diagnostics.
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