Abstract
Here, we report the controlled assembly of SWCNT–GFP hybrids employing DNA as a linker. Two distinct, enriched SWCNTs chiralities, (6,5), (7,6), and an unsorted SWCNT solution, were selectively functionalized with DNA and hybridized to a complementary GFPDNA conjugate. Atomic force microscopy images confirmed that GFP attachment occurred predominantly at the terminal ends of the nanotubes, as designed. The electronic coupling of the proteins to the nanotubes was confirmed via in-solution fluorescence spectroscopy, that revealed an increase in the emission intensity of GFP when linked to the CNTs.
Highlights
Dispersed SWCNTs (45 μL) were mixed with vortexed (7:3) solution (140 μL) and an aqueous solution of 10% polyvinyl pyrrolidone (PVP; 0.33 μL; 40 kDa), after which the combined solutions were vortexed for 30 s and centrifuged for 2 min at 13,000 rpm to separate the solution into two phases
green fluorescent protein (GFP) and SWCNTs, irrespective of which chiAs an additional control, the GFPDNA conjugate was hybridized to an excess amount rality is used
The GFPDNA conjugate was hybridized to an excess amount of the complementary sequence toassembly assess ifof duplexed
Summary
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