DNA DETECTION OF CAPRIPOXVIRUSES BY LOOP ISOMETRIC AMPLIFICATION BASED ON THE p32 / ld121 GENE REGION

Abstract

Bovine nodular dermatitis (Bovine lumpy skin disease) is a transmissible disease of cattle (Bos indicus and B. taurus) and Asian water buffaloes (Bubalus bubalis), that has been classified as a notifiable disease by the World Organization for Animal Health. Bovine lumpy skin disease virus belongs to the Poxviridae family. The poxviridae family can be divided into 2 subfamilies: Chordopoxviridae, which infects vertebrates, and Entopoxviridae, which infects insects. The causative agent of lumpy skin disease belongs to the subfamily Chordopoxviridae, the genus capripoxvirus, that also includes the causative agent of sheep and goat pox virus [1,2]. The genomes of goat and sheep pox viruses are very similar to the genome of bovine lumpy dermatitis virus. They have about 97% nucleotide identity. All genes for sheep pox and goat pox viruses are evident in bovine lumpy disease. The work was carried out using bioinformative and molecular genetic research methods. We have developed a technique for detecting capripoxvirus DNA by the method of isothermal loop amplification of the p32 / ld121 gene region under conditions of 30-40 min. amplification at 60 ° C. The developed technique is sensitive (analytical sensitivity corresponds to the activity of the virus 2 lg TCID50 / ml), specific and reproducible, and the developed primers do not hybridize with heterologous DNA templates.