DNA breathing dynamics under periodic forcing: Study of several distribution functions of relevant Brownian functionals.

Abstract

In this paper, we study DNA breathing dynamics in the presence of an external periodic force by proposing and inspecting several probability distribution functions (PDFs) of relevant Brownian functionals which specify the bubble lifetime, reactivity, and average size. We model the bubble dynamics process by an overdamped Langevin equation of broken base pairs on the Poland-Scheraga free energy landscape. Introducing an effective time-independent description for timescales larger than T[over ̃]=2π/ω (where ω is the frequency of external periodic force) and using an elegant backward Fokker-Planck method we derive closed form expressions of several PDFs associated with such stochastic processes. For instance, with an initial bubble size of x_{0}, we derive the following analytical expressions: (i) the PDF P(t_{f}|x_{0}) of the first passage time t_{f} which specifies the lifetime of the DNA breathing process, (ii) the PDF P(A|x_{0}) of the area A until the first passage time, and it provides much valuable information about the average bubble size and reactivity of the process, and (iii) the PDF P(M) associated with the maximum bubble size M of the breathing process before complete denaturation. Our analysis is limited to two limits: (a) large bubble size and (b) small bubble size. We further confirm our analytical predictions by computing the same PDFs with direct numerical simulations of the corresponding Langevin equations. We obtain very good agreement of our theoretical predictions with the numerically simulated results. Finally, several nontrivial scaling behaviors in the asymptotic limits for the above-mentioned PDFs are predicted, which can be verified further from experimental observation. Our main conclusion is that the large bubble dynamics is unaffected by the rapidly oscillating force, but the small bubble dynamics is significantly affected by the same periodic force.