Abstract
Cissus quadrangularis L. is a well-known plant used in Ayurveda. It is also called Asthisamharaka. The etymology of its name indicates its use in the healing of the fractured bone. It is a widely growing plant belonging to the family of vitaceae. C. quadrangularis (Family: Vitaceae) is commonly distributed throughout the hotter parts of India and Sri Lanka. C. quadrangularis is mentioned in classical literature like Bhava Prakash and Chakra Dutta. The present study design to compare three different varieties of C. quadrangularis which includes the species of quadrangular, round and flat types are used to study DNA barcoding marker and phylogenetic analysis. Whole-genome was isolated from the stem parts of three varieties of C. quadrangularis ; quadrangular, round and flat. The PCR amplification of DNA barcoding markers (rbcl, mat k and ITS) was analyzed by DNA electrophoresis. The DNA barcoding markers and next-generation sequencing are able to identify the intraspecies genetic variations among these closely related plant varieties of C. quadrangularis of quadrangular, round and flat.
Highlights
Asthisamharaka (C. quadrangularis) is a well known Ayurvedic drug for its effect on the reunion of a fractured bone
We have found the amplification of the gene was successful in rbcl gene region, Mat K gene was not amplified when compared with rbcl gene
This shows that these three varieties are only of same species varieties i.e of C. quadrangularis In phylogenetic tree analysis we have found that C. quadrangularis round variety was closely related with that of quadrangular variety and distance relationship with flat
Summary
Asthisamharaka (C. quadrangularis) is a well known Ayurvedic drug for its effect on the reunion of a fractured bone. Though in classical literature no varieties have been mentioned, in various geographical regions of Andhra Pradesh varieties of C. quadrangularis is classified to compare three different varieties of Cissus which includes the types of quadrangular, round and flat types are observed. Even studies denote that taxonomically quadrangular and flat variety do not have much difference[2]. Though there is a difference of opinions regarding the identification of flat and round varieties majority consider these as C. quadrangularis varieties and to be further confirmed and differentiated from C. repens and C. repanda[3]. The study is designed to evaluate the DNA barcoding markers of rbcl, mat k and ITS universal genes and next-generation sequencing to explore the intraspecies genetic variations among these closely related three varieties of C. quadrangularis
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