Abstract
Background: Datura is a well-known plant used in Ayurveda. It is a widely growing plant from Solanaceae family, attributed to poisonous and medicinal values. It is used in the treatment of various skin diseases, fever, etc., It is also used for external application and rarely for oral administration. Objectives: The present study aims to compare three different varieties of Datura which include the species of Datura innoxia Mill., Datura metel L. and Datura arborea are used to study molecular marker and phylogenetic analysis. Materials and Methods: Whole-genomic DNA was isolated from the leaves of Datura and the polymerase chain reactions amplification of DNA barcoding markers are rbcl, mat k, and internal transcribed spacer-4 and 5 were analyzed by 0.8% agarose gel electrophoresis. Results: The DNA barcoding markers and next-generation sequencing are able to identify the interspecies genetic variations among these closely related plant varieties of Datura. Conclusion: The interspecies genetic variations among these closely related three species of D. innoxia, metel, and arborea was closely related with Datura stramonium isolate NN003 chloroplast genome similarity of 98%, 99%, and 99%, respectively.
Highlights
Datura is mentioned in ancient classical literature like Charaka by the name of Kanaka.[1]
The aim of the present study is to evaluate the molecular identification of rbcl, mat k, and internal transcribed spacer (ITS) 4 and 5 universal genes and ‐generation sequencing (NGS) are used to explore the interspecies genetic variation among these closely related three plant varieties
Due to the good quality of genomic DNA concentrations in all three varieties of Datura was used as a DNA template for rbcl, mat k, and ITS‐4 and 5 for further molecular marker analyses
Summary
Datura is mentioned in ancient classical literature like Charaka by the name of Kanaka.[1]. Bhavamishra described Datura leaf externally for the treatment of lice, for analgesic and anti‐inflammatory actions.[2] Vangasena described Datura seeds are used after purification processes by boiling in cow milk and shade dried in the room temperature and used it for filariasis.[3] Datura is known by the name thorn apple is a poisonous herb. It is used in many Ayurvedic medicines after the purification method.[4] Leaves of Datura are used in alopecia, pain in the breast, tumor, rheumatism, and in inflammation and its flowers are anti-asthmatic.
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