Abstract
DNA barcoding is increasingly used to obtain taxonomic information about unidentified organisms. DNA barcoding involves sequencing a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene, “DNA barcodes,” from taxonomically unknown specimens and performing comparisons with a library of DNA barcodes of known taxonomy. In this article, we provide beginners with step-by-step instructions for (1) converting raw DNA sequences into clean DNA barcodes (sequence editing, sequence alignment), and (2) commonly used tools for DNA barcode comparisons (to assign taxonomic names to DNA barcodes, and to cluster DNA barcodes into Operational Taxonomic Units).
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