Abstract
A novel quantum dots (QDs) ECL biosensor for the detection of lysozyme was developed. Lysozyme was first incubated with probes immobilized at Au electrode in order to form the aptamer-lysozyme bioaffinity complexes. And the free probes were hybridized with the 5′-biotin modified cDNA oligonucleotides to form double-stranded DNA (ds-DNA) oligonucleotides. Avidin-QDs were bound to these hybridized cDNA through the biotin-avidin-system. The ECL signal of the biosensor was responsive to the amount of QDs bonded to the cDNA oligonucleotides, which was indirectly inverse proportional to the combined target protein.
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