Abstract
A survey of mold strains for the acylase property of asymmetric hydrolysis of N-acylated DL-amino acids was undertaken. Hundred and nineteen strains of Aspergillus and twenty strains of Penicillium were cultured on solid medium, wheat bran, and the extracts of the culture medium were assayed for the acylase activity. Acetyl-DL-glutamic acid hydrolysis test with the acylase was conducted by 24 hrs. incubation at pH 5.8 and 37° The procedure of determination of the liberated amino acid was modified from the method of STEIN and MOORE and the activity was expressed by the amount of the liberated amino acid (Table 2). A number of strains of Aspergillus and several strains of Penicillium produced sufficient acylase activity. The greatest activity was obtained by five strains of A. tamarii and each strain of A. cryzae, P. vinaceous, P. oxalicum var. pectinopcrum nov. and P. corymbiferum. The presence of calcium carbonate in the survey medium for Penicillium was found to be necessary for enzyme production to prevent the pH drop of the medium and to result the greatest enzyme yields (Table 1).
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