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Abstract
To better understand the roles of microorganisms in oil production, diversity and distribution of microbes in Jilin oilfield was studied. Firstly, chromosomal DNA wassuccessfully extracted directly from crude oil samples. Diversity and distribution of microbes was then analyzed based on 16S rDNA libraries. There were totally 21 OTUs were obtained through 16S rDNA sequencing. Of those OTUs, 13 are bacteria in whichProteobacteriais the major family, while 8 are archaea in whichMethanomicrobiais the dominant. Finally, SRB was found in all samples by amplifying theapsAgene using PCR. SRB found in Jilin oil samples belong toδ-Proteobacteria.
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