Abstract
The microbial resources in mangrove wetland ecological system are abundant because of their special characteristics. However traditional methods of isolation and culture alone cannot analyze the microbial diversity fully and so, in this study, a 16S rDNA library was constructed to analyze microbial diversity in the Fugong mangrove of the Jiulong River Estuary, Fujian Province, China. The total sediment DNA was extracted, a 16S rDNA library constructed, and the clones analyzed using the restriction fragment length polymorphism (RFLP) method. The 16S rDNA sequences of 50 clones which had a higher display frequency in the RFLP analysis were blasted with the sequences in GenBank. The results showed that the highest similarity of the sequence in clones was 100%, while the lowest was 88%. The dominant microbes from mangrove sediments in the 16S rDNA library belonged to the Proteobacteria (70%) including α-proteobacteria (6.0%), γ-proteobacteria (22.0%), δ-proteobacteria (10.0%) and ε-proteobacteria (32.0%). The other microbes were Bacteroidetes (8.0%), Planctomycetacia (2.0%), Actinobacteria (2.0%) and Verrucomicrobia (2.0%). Additional uncultured microorganisms as well as those whose classification information was unclear were also detected (16.0%). The results of this study indicated that more objective and comprehensive information of microbial diversity in mangrove wetland ecological system had been obtained. There is abundant microbial diversity and a large amount of unknown microbial resources in mangrove wetland ecological system, which could have a very important potential, and so there should be more research to explore and utilize these microbial and functional gene resources in mangrove wetland ecological system.
Highlights
The mangrove forest is a woody community that can be periodically submerged in seawater in the intertidal zone of tropical and subtropical regions [1]
The fragment size of 1.5kb was amplified in 16S rDNA of purified samples using PCR in which the primers were Eubac27F and Eubac1492R. 500 white clones were randomly picked out to the LB plates with ampicillin after blue/white screening, incubated at 37 °C for 16 hours, before being stored at 4°C
The 250 DNA inserts from recombinant clones that were randomly selected in the 16S rDNA library were reamplified by PCR using the vector primers M13-47 and RV-M
Summary
The mangrove forest is a woody community that can be periodically submerged in seawater in the intertidal zone of tropical and subtropical regions [1]. The structure and function of this ecological system combines marine and terrestrial properties and has its own unique features: (1) the soil has deoxidized characteristics; (2) there is a higher salinity and salinization trend; (3) a lower pH value and higher acidity; and (4) a higher content of organic matter [2]. These systems are critical areas where biodiversity and valuable biological resources are very rich [3]. Knowledge of bacterial diversity in mangrove sediments is important for understanding how mangrove ecosystems function
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