Divergent changes of carbon and nitrogen in the density fractions of soil organic matter after revegetation in the Tengger Desert, north China

Abstract

AbstractRevegetation is an effective measure to enhance soil organic carbon (OC) and nitrogen (N) storage in drylands, but the underlying processes remain poorly understood. Based on density fractionation, the free light fraction (fLF), the occluded light fraction (oLF), and a heavy fraction (HF) were extracted from a chronosequence of revegetated sites aged 10, 22, 34, 48, and 65 years. A mobile sand dune (MSD) was used as the reference site (aged 0 years). The OC and N contents of different fractions and the bulk soils were determined to evaluate the impacts of revegetation and clarify post‐revegetation stabilization mechanisms for the OC and N. The results showed that the dry mass of fLF and oLF in established shrublands were 17.16–31.30 and 17.68–44.87 times greater than those in the MSD, respectively, while the amount of HF decreased by 1.09%–2.51% in 65 years. The contents and stocks of OC and N and C:N ratio in each fraction significantly increased over time but decreased with soil depth, and these three variables decreased sequentially from fLF to oLF to HF. The OC and N contents and their ratios in each fraction were positively and linearly correlated with their corresponding values in the bulk soil. The OC and N percentages contained in the fLF and oLF increased significantly, but those in the HF decreased with increasing site age. Despite this, more than 55.08% of OC and 80.59% of N were remained in the HF across all the sites, indicating that the enhancement of OC and N stocks in SOM following revegetation were mainly derived from an increase in OC and N in protected fractions. Biological and physicochemical factors including microbial biomass C and N, the availability of N, P, and K, bulk density, and clay and silt contents, were the key factors regulating the OC and N dynamics in the density fractions. The initial OC and N accumulation after revegetation occurs mainly in the HF, while vegetation development shifts the OC and N from stable to labile pools. The OC and N accumulation in revegetated soils is attributed to the changes in both the LF and HF whereas OC and N in the light fraction become increasingly important over time. Stabilization by forming aggregates and mineral associations may dominate the long‐term OC and N sequestration in restored ecosystems.