Divergences of soil carbon turnover and regulation in alpine steppes and meadows on the Tibetan Plateau

Abstract

The grasslands of the Tibetan Plateau store approximately 2.5% of global soil organic carbon (SOC) and considerable soil inorganic carbon (SIC) and have the potential to become a vast carbon source or sink as climate change progresses. However, the soil carbon (C) sequestration mechanisms that occur across large-scale natural gradients remain unclear. Here, humic substances (HS) were utilized to trace soil C turnover at 0–20 cm, and we compared divergences among three main grassland types (alpine meadow, alpine steppe, and artificial plantation) using structural equation modeling (SEM). The results showed that the alpine meadows sequestered the most soil C (63.99 ± 4.41 g kg−1 SOC and 4.11 ± 0.63 g kg−1 SIC), sequestering 2–3 times more than the alpine steppe ecosystems (19.78 ± 1.98 g kg−1 SOC and 9.21 ± 0.66 g kg−1 SIC). The alpine steppe and artificial plantation regions have strong C sink potential due to their low C/N ratios (P < 0.05). Importantly, SIC played an important role in the alpine steppes, accounting for nearly 26–37% of soil C. The ratios of recalcitrant HS to SOC were estimated as 46.50%, 65.09%, and 78.17% in the alpine meadow, alpine steppe, and artificial plantation ecosystems, respectively, indicating that SOC in the alpine meadow was the most sensitive to climate change. Fulvic acid (FA) accounted for 50.86% of SOC in the 0–20-cm interval, contributing most to the formation of SOC in all vegetation types. In addition, in contrast to climatic controls on soil C turnover in the alpine meadow, climate conditions rarely controlled C turnover in the alpine steppe. Moreover, sand and silt were the main soil minerals involved in C turnover in alpine meadow and alpine steppe ecosystems, respectively. Our study improves understanding of the mechanism by which soil C sinks form on the Tibetan Plateau under warming and wetting conditions.