Diurnal pattern of aphid feeding and its effect on cotton leaf physiology

Abstract

Cotton aphid ( Aphis gossypii G.) populations seemed to fluctuate over the past years in cotton ( Gossypium hirsutum L.) perhaps as a result of excessive use of insecticides for controlling more problematic pests. Contradictory plant responses have been observed depending upon the aphid/plant system, and it is unclear if cotton aphids, abiotic stress or both are responsible for cotton yield reduction in aphid-infested fields. Our objectives were to investigate the diurnal changes in the physiology of cotton leaves following aphid herbivory, and the diurnal pattern of aphid feeding. The experiment was conducted in a growth chamber using the cotton cultivar ‘Stoneville 474’. Leaves of the same age and size were infested with wingless adults plus nymphs. Cotton aphids were allowed to increase in numbers without restriction for 9 days, after which the amounts of carbohydrates in aphid-honeydew, and the number of honeydew droplets excreted per aphid were measured. Photosynthetic rates, dark respiration rates and foliar non-structural carbohydrates were measured. The amount of individual carbohydrates found in the honeydew was significantly different with time. The total amount of carbohydrates excreted per aphid within a 24-h period averaged 2.5 μg. The number of honeydew droplets excreted per aphid varied significantly from time to time period. Cotton aphids did not significantly alter photosynthesis or respiration rates or non-structural carbohydrates on leaves. Aphid populations of approximately 300 per leaf on the 9th day of infestation did not appear to significantly alter the physiology of cotton leaves.