Diuretic Activity and Acute Toxicity of Combination Eurycoma longifolia Extract and Irbesartan

Abstract

“ Pasak bumi ” ( Eurycoma longifolia Jack.) has been used traditionally for aphrodisiac , antimalaria, activit ies as anticancer, antidiabetic , and antihypertension. E. longifolia can be used with synthetic hypertensive drugs like irbesartan. This study was to investigate the diuretic effect of E . longifolia extract and its combination with irbesartan in normal rats, determinate the LD 50 value and the toxic effect that influence the organ weight and histopathology of mice. Aqueous extracts of E. longifolia ( 40 mg/kgBB ) and its combination with irbesartan (20, 40, 80 mg/kgBB) were administered orally to experimental rats strain Sprague Dawley . The con c entration of sodium and potassium from urine were measured with AAS. The data was analy z ed with ANOVA one way. The acute toxicity test was determinated by Weil method. The dose of combination E. longifolia extract - irbesartan were 1000 mg, 2000 mg, 4000 mg and 8000 mg/kg. T he observation was carried out in 14 day including clinical signs, percentages of death and histopatology of mice organs. The result showed that all of tested group have significant diuretic activity (p<0.05) compar ed with control group, there ’s no significant difference between single dose extract of E.longifolia (40 mg/kgBB ) and irbesartan (40 mg/kgBB) but the combination of both have lower activity. The combination of E. longifolia extract with irbesartan have LD 50 value 23 . 951 g/kg ( categorized as non toxic compound ). The result of histopathological showed that there ’re no significant pathologic effects in the kidney, liver and heart. Keyword s : A cute toxicity , d iuretic, E. l ongifolia , irbesartan, herb-drug interaction