Dittrichia viscosa L. Ethanolic Extract Based Ointment with Antiradical, Antioxidant, and Healing Wound Activities.

Wafa Rhimi,Ahmed Rejeb,Raoudha Hlel,Mouldi Saidi,Issam Ben Salem,Abdennacer Boulila

doi:10.1155/2019/4081253

Abstract

Dittrichia viscosa which belongs to the Asteraceae family is frequently used to treat hematomas and skin disorders in Mediterranean herbal medicine. This study aims to validate its antioxidant effects and its potential on healing wounds. The ethanolic extract of D. viscosa leaves was formulated as 2.5% and 5% (w/w) in ointment bases on the beeswax and sesame oil. During this study, the ethanolic D. viscosa extract, ointments containing 2.5% and 5% of D. viscosa extract, and the vehiculum were assessed for their total phenol content (TPC), caffeoylquinic acid content (CQC), and antioxidant activities using complementary methods (TAC, the DPPH, ABTS, FRAP, and the BCB). The effects on wound healing of obtained ointments were evaluated by excision of the wound in a mice model for 12 days. Subsequently, the excised wound areas were measured at the 3rd, 9th, and 12th days. The skin tissues were isolated for histological studies. The ointments containing D. viscosa extract (2.5%, 5%) possessed a considerable TPC, CQC, radical scavenging potential, and antioxidant activities compared to the vehiculum. Treated animals with ointments containing D. viscosa extract at 2.5% and 5% showed almost and totally healed wounds compared to the vehiculum and control groups, evidenced by good skin regeneration and reepithelialization. The present work showed the role of D. viscosa antioxidants exerted by its polyphenolic compounds, in particular, caffeoylquinic acids, in enhancing wound healing.

Highlights

The research to ensure a good quality of wound closure and scarless healing remains a health preoccupation until today [1, 2]
This assay based on the measure of the discoloration of ß-carotene during the oxidation of linoleic acid at 50∘C of temperature. 0.2 mg of ß-carotene, 20 mg of linoleic acid, and 200 mg of tween 40 were dissolved in 0.5 mL of chloroform
UV spectra, and retention time of each peak of high performance liquid chromatography analysis (HPLC)-photodiode array detector (PDA)-ESI-MS/MS data, 29 phenolics were identified in ethanol extract of D. viscosa (Table 1)

Summary

Introduction

The research to ensure a good quality of wound closure and scarless healing remains a health preoccupation until today [1, 2]. An excess of ROS level causes an imbalance between cellular production of free radicals (oxidants), and antioxidant defenses mechanisms, augmentation in inflammatory response, and inhibition of the wound repair [5, 6]. Antioxidants are scavengers molecules which are indispensable for neutralization of free radicals and for remediation of ROS damage during healing process [3, 6]. In this sense, plants extracts are emerging as a rich source of active compounds (i.e., triterpenes, flavonoids, polyphenolic, and tannins) for their pertinent properties to prevent from (i) accumulation of free radicals, (ii) oxidation of lipid, and (iii) inhibition of inflammatory disease.

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