Comparison of Different Extraction Solvents for Characterization of Antioxidant Potential and Polyphenolic Composition in Boletus edulis and Cantharellus cibarius Mushrooms from Romania.

Melinda Fogarasi,Sonia Ancuța Socaci,Szabolcs Fogarasi,Carmen Socaciu,Claudiu-Dan Sălăgean,Floricuța Ranga,Maria-Ioana Socaciu,Dorin Țibulcă,Anca Corina Fărcaș,Cristina Anamaria Semeniuc

doi:10.3390/molecules26247508

Abstract

Edible mushrooms are well-known for their nutritional benefits and low energy density. In addition, mushroom extracts contain various bioactive compounds that account for their antioxidant activity; the applied extraction conditions influence the extraction efficiency of such compounds. Therefore, this study investigates the effects of four extractants on the content of polyphenols and antioxidant properties of Boletus edulis and Cantharellus cibarius mushrooms, aiming to optimize the extraction process. Powders of B. edulis and C. cibarius mushrooms were subjected to extraction with acidic water (10% CH3COOH), ethanol/water/acetic acid (15:76.5:8.5, v/v/v), hexane, and diethyl ether to measure their total phenolic content (TPC), total flavonoid content (TFC), and Trolox equivalent antioxidant capacity (TEAC). Furthermore, the level of individual polyphenolic compounds in these extracts was quantified using an HPLC-DAD-ESI-MS method. Results showed that the type of solvent significantly influenced the TPC and TEAC of mushroom powder but insignificantly influenced the TFC. A very strong positive correlation was found between TPC and TEAC, but no correlation was found between TFC and TEAC or TPC and TFC. Acidic water extracted the highest amount of polyphenolic compounds from these mushroom powders. Therefore, the aqueous extract showed the highest TPC and strongest antioxidant activity. Thus, acidic water is recommended for polyphenol analysis in B. edulis and C. cibarius mushrooms.

Highlights

Proximate analysis results of B. edulis powder revealed a mean value of 26.5 ± 0.190%
This study reveals that B. edulis and C. cibarius mushrooms are sources of polyphenolic compounds and exhibit antioxidant activities
All authors have read and agreed to the published version of the manuscript

Summary

Introduction

The tradition of wild edible mushroom consumption has existed for centuries in many European countries [1]. Two hundred sixty-eight edible wild mushrooms are allowed for commercialization in Europe; the five top-selling edible fungi are Boletus edulis, Cantharellus cibarius, Lactarius deliciosus, Morchella esculenta, and Agaricus campestris [2]. Boletus edulis (porcini mushrooms) and Cantharellus cibarius (chanterelle) are among the most consumed wild mushrooms in Romania because of their availability in large quantities in this territory. These mushrooms are consumed fresh, dehydrated, or preserved in brine, vinegar, and oil

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Conclusion