Abstract
Ten diterpenoid alkaloids, including eight aconitine-type C19-diterpenoid alkaloids and two hetisine-type C20-diterpenoid alkaloids, were isolated from the secondary roots of Aconitum carmichaeli Debx., known as “Fuzi” in Chinese traditional herbal medicine. Their structures were established on the basis of their spectroscopic data and comparison with those of the literature. Among these alkaloids, chasmanine, oxonitine and 15-acetylsongoramine were isolated for the first time from this medicinal plant. The cytotoxic activity of the alkaloids were tested against several cell lines by the MTT method in which aconitine, hypaconitine, mesaconitne and oxonitine were found to strongly inhibit the growth of the HePG2 cell line, which showed that the existence and quantity of the ester groups have a significant influence on the cytotoxicity of the diterpenoid alkaloids.
Highlights
The secondary roots of Aconitum carmichaeli Debx. (Ranunculaceae), a famous Chinese traditional herbal known as “Fuzi”, found mainly distributed in Jiangyou City of Sichuan Province in China, have long been used as an analgesic, anti-inflammatory and anti-tumor agent [1]
In order to find more anti-tumor active substances, the secondary roots of Aconitum carmichaeli were phytochemically and pharmacologically investigated to obtain eight aconitine-type C19-diterpenoid alkaloids: aconitine (1), chasmanine (2), crassicauline A (3), oxonitine (4), deoxyaconitine (5), hypaconitine (6), mesaconitine (7), senbusine A (8), and two atisine-type C20-diterpenoid alkaloids, songoramine (9), 15-cetylsongoramine (10)
The ten isolated compounds were identified as aconitine (1), chasmanine (2), crassicauline A (3), oxonitine (4), deoxyaconitine (5), hypaconitine (6), mesaconitine (7), senbusine A (8), and two atisine-type C20-diterpenoid alkaloids, songoramine (9) and 15-cetylsongoramine (10), by comparison of their 1H- and 13C-NMR spectroscopic data with those reported in the literature
Summary
The secondary roots of Aconitum carmichaeli Debx. (Ranunculaceae), a famous Chinese traditional herbal known as “Fuzi”, found mainly distributed in Jiangyou City of Sichuan Province in China, have long been used as an analgesic, anti-inflammatory and anti-tumor agent [1]. The secondary roots of Aconitum carmichaeli Debx. The anti-tumor activity of these diterpenoid alkaloids with complex structures have attracted increasing interest [3,4]. Forty-seven alkaloids, including forty-three C19-diterpenoid alkaloids and four C20-diterpenoid alkaloids have been isolated from the Aconitum carmichaeli Debx [1,5]. In order to find more anti-tumor active substances, the secondary roots of Aconitum carmichaeli were phytochemically and pharmacologically investigated to obtain eight aconitine-type C19-diterpenoid alkaloids: aconitine (1), chasmanine (2), crassicauline A (3), oxonitine (4), deoxyaconitine (5), hypaconitine (6), mesaconitine (7), senbusine A (8), and two atisine-type C20-diterpenoid alkaloids, songoramine (9), 15-cetylsongoramine (10). We report the extraction, isolation, structural elucidation and cytotoxicity of diterpenoid alkaloids from the well-known.
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