Abstract
Deionized water and glucose without yeast and with yeast (Saccharomyces cerevisiae) of optical density OD600 that ranges from 4 to 16 has been put in the ring electrode region of six different types of impedance biochips and impedance has been measured in dependence on the added volume (20, 21, 22, 23, 24, 25 µL). The measured impedance of two out of the six types of biochips is strongly sensitive to the addition of both liquid without yeast and liquid with yeast and modelled impedance reveals a linear relationship between the impedance model parameters and yeast concentration. The presented biochips allow for continuous impedance measurements without interrupting the cultivation of the yeast. A multiparameter fit of the impedance model parameters allows for determining the concentration of yeast (cy) in the range from cy = 3.3 × 107 to cy = 17 × 107 cells/mL. This work shows that independent on the liquid, i.e., DI water or glucose, the impedance model parameters of the two most sensitive types of biochips with liquid without yeast and with liquid with yeast are clearly distinguishable for the two most sensitive types of biochips.
Highlights
There is an ongoing search towards disturbing-free determination of biomaterial concentration, e.g., to gain better control over the growth of cell cultures
S. cerevisiae cells used in this research were cultivated overnight in 50 mL Erlenmeyer flasks in sterile medium under shaking
In this work we analyzed the interaction between the surface of inner ring region of the top electrode of the impedance biochips, i.e., a thin silica layer on Si P-N junction, and the yeast cells
Summary
There is an ongoing search towards disturbing-free determination of biomaterial concentration, e.g., to gain better control over the growth of cell cultures. Standard optical microscopy investigations disturb cell growth. This method is less convenient and time consuming (not suitable for long term measurements and number determination). The plate count agar (PCA) technique and counting under microscope would be highly time consuming and it always involves human errors. Such disadvantages call for more adequate electrical analysis methods, e.g., microfluidic impedance cytometer [1,2,3,4] and impedance biochips, which have been used to count and discriminate yeast cells on the basis of their dielectric and electrical properties
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