Abstract
Starches were extracted from sweet potatoes differing in stages of development, tissue zone and variety. A series of maltosaccharides produced after debranching each starch with isoamylase were analyzed using high performance anion exchange chromatography equipped with a pulsed amperometric detector. Using this method, the chain length [degree of polymerization (DP), 6-17] distributions of amylopectin molecules were characterized to evaluate the influence of genetic and physiological factors on the properties of sweet potato amylopection. No significant changes were observed in the chain length distributions of amylopectins at various stage of development. In addition, no significant differences were observed in the chain length distributions of amylopectins from different tissue zones. Therefore, it was suggested that physiological conditions have very little effect on the molecular structure of sweet potato amylopectin. Only small variations in the chain length distributions of amylopectins were observed within 31 varieties of sweet potato. The proportion of short chains in the range of DP of 6-10 ranged from 28.7% (Tokai 5) to 37.6% (Kyushu 91) within these varieties.
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