Abstract
The structure of endosperm amylopectin was compared between two rice varieties, Kinmaze (subspecies japonica) and IR36 (subspecies indica), as well as their waxy mutants, all grown under controlled temperature. The distinct varietal difference in chain length distribution of amylopectin was confirmed by high performance anion-exchange chromatography equipped with pulsed amperometric detection. Amylopectin from Kinmaze contains more very short chains with degree of polymerization (DP) between 6 and 10 and less chains with DP from 13 to 22 than amylopectin from IR36, while there is little difference in the distribution of longer chains with DP > 24 between the two varieties. Waxy mutation had little effect on chain length distribution of endosperm amylopectin. The temperature during grain-filling affected the chain length distribution of amylopectin in both varieties in a similar way; grain-filling at lower temperatures lead to an increased proportion of chains of DP 6—13 and decreased the percentage of chains with DP 20—27 and DP 44—54. However, the temperature-dependent changes in chain length distribution of amylopectin were within the range of varietal difference between Kinmaze and IR36. These results strongly suggest that factors regulating the varietal difference in patterns of chain length of amylopectin are dissimilar to those causing the temperature effects on amylopectin fine structure in rice endosperm.
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