Distributions of interstitial cells of Cajal in stomach and colon of cat, dog, ferret, opossum, rat, guinea pig and rabbit

Abstract

Electrical slow waves vary in prominence from one location to another in stomach and colon. If interstitial cells of Cajal are involved in generation of the slow waves, they might be more abundant in regions where slow waves are prominent than in regions where slow waves are small or absent. We calculated the density of distribution of interstitial cells of Cajal in colon and stomach of cat, dog, ferret, guinea pig, opossum, rabbit, and rat, using the zinc iodide-osmic acid reaction for light microscopy. The cells in the stomach were concentrated in the myenteric plexus and in the circular muscle layer. Along the greater curvature, cells were relatively sparse in the fundus, more frequent in the corpus and densest in the antrum. Along the lesser curvature, they were denser distally than proximally in all species except guinea pig. In the colon most cells lay with an axonal network at the circular muscle-submucosal interface. The axons were sparse in the cecum but uniformly conspicuous from the ileocolonic junction to the internal anal sphincter. The density of the interstitial cells of Cajal in cat, dog, opossum, and rabbit rose from a relatively low level in the cecum to a maximum in the mid-colon, declining toward the rectum. In guinea pig, rat, and ferret, the levels throughout the most proximal colon were high. Since gastric slow waves are absent from the fundus, diminutive in the body, and prominent in the antrum, the density of interstitial cells of Cajal in the stomach roughly parallels the prominence of slow waves. Colonic slow waves are most prominent in the right colon and mid-colon, and so the density distribution of interstitial cells of Cajal in the colon also roughly parallels the prominence of slow waves.