Distributions of Allelic Frequencies and Haplotypes of Two New STR Loci in a Chinese Han Population

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Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/d3s4014.htm. The PCR reaction volume for each locus was 37.5μL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data of population genetics and forensic science were analyzed using POWERSTATS program (3). The genotype distribution was analyzed for Hardy-Weinberg equilibrium