Abstract
Affinity chromatography for albumin has been coupled with electrothermal atomic-absorption spectrophotometry to determine the distribution of zinc between albumin and globulin ligands in normal human serum. The procedure is both simple and rapid and requires only 400 µl of serum for duplicate analyses. There is no alteration in the distribution of zinc between albumin and the globulin during the separation process and the total recovery of zinc from the column is quantitative, 98.6%. Albumin-bound zinc and globulin-bound zinc are determined with relative standard deviations of 4.5 and 5.9%, respectively. The distribution of zinc obtained is in very good agreement with that found using more complex techniques.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
