Abstract
Distribution of Two Types of Phosphoglyceric Acid Mutase, Diphosphoglycerate Mutase, and d-2,3-Diphosphoglyceric Acid
Highlights
Assay: The samples were tested for phosphoglyceric acid mutase with 3-PGA free from 2,&DPGA and in the presence of 4 x 1O-5 M 2,3-DPGA as previously described [4, 5]
We observed previously that the phosphoglyceric acid mu&se from several tissues, with the exception of that from wheat germ, was stimulated by 2,&DPGA [6], and we found when studying the enzyme diphosphoglycerate mutase that there appeared to be a correlation in several sources between the activity for 2,3DPGA biosynthesis and the stimulation by 2,3-DPGA on phosphogiyceric acid mutase from the same tissue
Diphosphoglycerate mutase activity was measured by the assay method A described in the companion paper [1]
Summary
Assay: The samples were tested for phosphoglyceric acid mutase with 3-PGA free from 2,&DPGA and in the presence of 4 x 1O-5 M 2,3-DPGA as previously described [4, 5]. Diphosphoglycerate mutase activity was measured by the assay method A described in the companion paper [1]. Phosphoglyceric acid mutase in the absence of 2,3DPGA
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