Distribution of repeated DNA families in the human genome

Abstract

By means of restriction enzymes analysis and molecular hybridization, the distribution of repeated DNA families has been studied in the different DNA components into which the human genome can be fractionated by density gradient techniques. Three classes of DNA molecules have been analyzed: i) an homogeneous DNA component (satellite-like sequences; ϱ=1.696g/cm 3, 3% of total DNA, AT repeated ), ii) AT rich (ϱ=1.698g/cm 3, 30% of total DNA, AT main-band ) and GC rich (ϱ=1.708 g/cm 3, 6% of total DNA, GC main-band ) DNA components. By this approach we have observed that Sau3A digestion of GC main-band gives rise to two bands of 75bp and 150bp, absent or under-represented in both AT rich DNA components. A preliminary characterization of these DNA fragments suggests that they contain one or more families of repeated sequences which fail to hybridize to EcoRI, HindIII and AluI families of repeats. In addition, we have observed that EcoRI sequences (α-RI DNA) are under-represented in GC main-band and show the same clustered organization in both AT rich DNA components.