Abstract
Extracts obtained by manual grinding of the mycelium and microconidia of Fusarium oxysporum were fractionated into four particulate portions—designated P1, P2, P3 and P4—and into a soluble portion by differential centrifugation. Approximately 60% of the protein was recovered in the supernatant fraction whereas the ribonucleic acid content of the P3 and P4 particles was particularly high. The P4 particle fraction was composed of approximately 40% ribonucleic acid and 60% protein and contained a particle with a sedimentation constant of about 85 S. More than 70% of the deoxyribonucleic acid was recovered among the various particle types, but the deoxyribonucleic acid: protein ratio was highest in the P1 granules. Polysaccharides were particularly concentrated in the P3 fraction. On the basis of the distribution of bound hexosamine, the extracts appeared to be free of cell wall fragments. Several methods of disruption of the fungus hyphae were examined.
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