Distribution of pathogenic bacteria and drug sensitivity analysis of bloodstream infection

Abstract

Objective To analyze the distribution and drug sensitivity of pathogenic bacteria in bloodstream infection, and to provide drug using evidences for clinical application. Methods Using French bioMerieux company’s BACT/ALERT 3D full-automatic blood culture instrument with its accessory culture bottle to culture the bacteria. The drug susceptibility of bacterial strains were identified and tested by VITEK-2 compact full-automatic microbiology analysis system. Results A total of 540 strains of pathogenic bacteria were isolated from 6 227 blood culture specimens, the positive rate was 8.7%. Among them, there were 373 strains of gram-negative bacteria, accounting for 69.1%; 149 strains of gram-positive bacteria, accounting for 27.6%; and 18 strains of fungi, accounting for 3.3%. The top three pathogenic bacteria detected were Escherichia coli (36.3%), Klebsiella pneumonia (17.2%), and Staphylococcus (18.4%). According to drug susceptibility, the sensitive rates of Escherichia coli and Klebsiella pneumoniae to imipenem, cefotetan, piperacillin-tazobactam, and amikacin were all over 92%. Drug resistance of coagulase negative staphylococcus was more serious than staphylococcus aureus. No staphylococcus which was resistant to vancomycin and linezolid was detected. Conclusions The distribution of bloodstream infection pathogenic bacteria is widespread, mainly on gram-negative bacteria. And the situation of drug resistance is serious. We should pay attention to the blood bacteria culture and drug sensitivity analysis, and choose antibacterial agents reasonably according to drug sensitivity tests. Key words: Bloodstream infection; Distribution of pathogenic Bacteria; Drug sensitivity analysis