Abstract
To study the distribution of the methoxyl groups, pectins (mainly from lemon peels) have been degraded extensively by purified pectin lyase. The resulting fragments were fractionated by gel permeation chromatography and high-pressure liquid chromatography (ion exchange). The chromatograms of the pectins were compared to those of ‘transesterified’ pectins in which a random distribution of the methoxyl groups is assumed. The results indicate that differences exist between natural pectins and transesterified pectins. In native pectins more isolated non-esterified galacturonate residues are present. The oligomer pattern (distribution of degree of polymerization) of the pectin lyase degraded pectins was also found to be different for native and transesterified pectins.
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