Immobilization of Purified Pectin Lyase from Pseudomonas putida onto Magnetic Lily Flowers (Lilium candidum L.) Nanoparticles and Applicability in Industrial Processes.

Esen Tasgin,Nazan Demir,Hayrunnisa Nadaroglu,Aynur Babagil

doi:10.3390/molecules25112671

Esen Tasgin, Nazan Demir + Show 2 more

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https://doi.org/10.3390/molecules25112671

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Abstract

Pectinases are an important class of enzymes distributed in many higher plants and microorganisms. One of these enzymes is pectin lyase which has an important role in industrial applications such as clarification of fruit juices. Pectin lyase was purified with 73% yield from Pseudomonas putida bacteria and was 220.7-fold using three phase precipitation technique. Molecular weight of purified pectin lyase was determined as 32.88 kDa with SDS-polyacrylamide gel electrophoresis. The pectin lyase was immobilized covalently via the L-glutaraldehyde spacer to the cellulosic structures of lily flowers (Lilium candidum L.). The immobilized enzyme was then magnetized by modifying with γ-Fe3O4 nanoparticles and determined the most appropriate immobilization conditions as pH 6 and 30 °C. Purified pectin lyase was connected to magnetized support material after 60 min at the rate of 86.4%. The optimum pH and temperatures for the free and immobilized pectin lyase was found to be 6.0 and 40 °C. pH and thermal stabilities of the free and immobilized pectin lyase enzyme have been preserved at high-low temperatures and pH. The structural characterization of the immobilized pectin lyase was performed by SEM, FT-IR, and XRD chromatographic analyses and it was observed that the support materials structure was appropriated to immobilization with pectin lyase and to modify with Fe3O4 nanoparticles.

Highlights

IntroductionInterest in biopolymers such as cellulose, starch, lignin, and pectin has been increasing
Interest in biopolymers such as cellulose, starch, lignin, and pectin has been increasing.Pectinases are a large group of enzymes that break down pectin polysaccharides in plant tissues through depolymerization and deesterification reactions and convert them into simpler molecules such as galacturonic acids
Pectin lyase purified from Pseudomonas putida bacterium was magnetized by using

Summary

Introduction

Interest in biopolymers such as cellulose, starch, lignin, and pectin has been increasing. Pectinases are a large group of enzymes that break down pectin polysaccharides in plant tissues through depolymerization and deesterification reactions and convert them into simpler molecules such as galacturonic acids. Pectinases are produced by a large number of organisms such as bacteria [1,2], fungi [3], and yeasts [4]. It has been reported that most of the pectin lyases studied so far have been obtained from microorganisms and have insufficient presence in plants and animals [5]. Pectinolytic enzymes obtained from microorganisms are of great interest because of their biotechnological potential and their role in industrial applications [6]. One of the most important enzymes in this group, pectin

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