Distribution of metals in tissues of captive and wild Morelet's crocodiles and the potential of metallothioneins in blood fractions as a biomarker of metal exposure.

Abstract

The distribution of Hg, Cd, Cu, and Zn in keratinized tissues, blood fractions, and excretory organs, and MTs in blood fractions and excretory organs was determined in captive, semicaptive, and wild Morelet's crocodiles and they were compared to select the most useful non-destructive tissues for the monitoring of metal exposure and to assess the potential of MTs as a biomarker. Our results indicate blood plasma, claws, and caudal scutes altogether are suitable tissues for xenobiotic metals exposure, with concentrations in blood plasma being an indicator of recent exposure, whereas concentrations in claws and caudal scutes are indicators of chronic exposure. Results in keratinized tissues suggest they are an important detoxification strategy in crocodiles, and claws presented the highest concentrations of metals in both captive (Hg=0.44±0.23μgg-1, Cd=11.10±5.89μgg-1, Cu=45.98±23.18μgg-1, Zn=124.75±75.84μgg-1) and wild populations (Hg=1.31±0.32μgg-1, Cd=26.47±21.15μgg-1, Cu=191.75±165.91μgg-1, Zn=265.81±90.62μgg-1). Thus, they are an appropriate tool for assessing metal exposure in populations where scutes clipping as a marking technique is not allowed, and their collection is less complicated than with other tissues. MTs are a suitable biomarker in blood plasma, whereas in erythrocytes detoxification processes might depend on hemoglobin, rather than MTs. Future studies should consider the implementation of these tools for the monitoring of wild populations.