Abstract
SYNOPSIS. Ochromonas malhamensis contains folate derivatives active for Streptococcus faecalis, Pediococcus cerevisiae and Lactobacillus casei. These activities increase about 6‐, 4‐ and 3‐fold, respectively, on treatment with chicken liver conjugase, establishing the presence of polyglutamyl folates in the organism. The nature of the folate derivatives as well as their functional groups has been ascertained by chromatography on DEAE cellulose columns and assay of the activities of the eluted fractions, before and after the conjugase treatment, using differential microbiologic assay technic. The folate complex has been resolved into 7 fractions corresponding to N10‐formyltetrahydrofolic acid, N5‐formyltetrahydrofolic acid, unsubstituted tetrahydrofolic acid, and 4 polyglutamyl folates. The implications of these findings are indicated.
Published Version
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