Abstract
Summary Flavanols, including their dimers and oligomers (proanthocyanidins), were identified in different tissues of Prunus avium L.using spectrophotometry and histochemistry combined with microscope photometry. Estimation by microscope photometry revealed the greatest amounts in the phloem rays, ray initials, and phelloderm. Small amounts were found in the phellem, xylem, and pith. Wounding of shoots stimulated the accumulation of flavanols, especially in the phloem rays, ray initials, and cambium. Phellogen and cambium of vigorously growing shoots contained varying levels of flavanols and their actual staining intensity was roughly similar when compared to the adjacent mature tissues. The uppermost 4 internodes of vigorously growing shoots accumulated high levels of flavanols without major differences among cortex, young phloem, procambium, and developing xylem. Only the large-vacuolized cells of the pith exhibited lower levels. Older internodes, as well as shoots grown under reduced light conditions, showed a marked reduction of flavanols in all tissues investigated.
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