Distribution of fatty acids in triacylglycerols from ungrafted Desi Mango (Mangifera indica) kernel lipids

Abstract

The lipids of ungrafted ‘Desi’ mango (Mangifera indica) kernel were extracted with chloroform/methanol, purified and triacylglycerols (82.6%) were separated by thin layer chromatography (TLC). The pure triglycerides are fractionated into five bands by 25% silver nitrate-impregnated thin layer chromatography (Ag-TLC). The composition and position of fatty acids at α, α′ and β-positions of fractionated/unfractionated triacylglycerols were determined by the use of pancreatic lipase, sodium tetraborate-impregnated thin layer chromatography (borate-TLC) and gas liquid chromatography (GLC). The highest percentage of the most saturated band was 55.6% which comprised of sn-1,3-disaturated-2-oleoglycerols and sn-1(3)saturated-2,3(1,2)-dioleoylglycerols. The oleic, linoleic and linolenic acids occupied β-position depending upon comparatively higher percentage of unsaturated fatty acids in specific fraction.