Abstract
Summary Purified preparations of IgG1, IgG2, IgA, IgM and low molecular weight antibody were obtained from serum and whey of Brucella positive cattle and tested for Brucella antibody activity in a variety of commonly used diagnostic tests. Antibody in all preparations except IgM exhibited antiglobulin enhancement. In contrast to IgG1, neither IgC2 nor IgA fixed complement, and IgM fixed complement at titres much lower than those obtained in the direct agglutination test. Antibody in IgM and IgA preparations lost most of its agglutinating activity after heating at 65°C for 15 min. There was a marked reduction in the titre of all preparations when the antigen of the Rose Bengal plate test was used in slide agglutination tests. It was evident that IgM and IgA antibody reacted more quickly in the milk ring test than the remaining antibody preparations. IgG2 and low molecular weight antibody appeared to have less affinity for the fat globule membrane than the antibody in the other classes of immunoglobulin.
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