Abstract
Perirenal adipose tissue (PrAT) is a visceral adipose tissue involved in the pathogenesis of obesity and cardiovascular diseases via neural pathways. However, the origins, morphological characterization, and resiniferatoxin (RTX)-susceptibility of sensory neurons that innervate rat PrAT are yet unclear. Using neural tracing, an injection of DiI (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate) into PrAT revealed that sensory neurons that innervate PrAT reside in T9-L3 dorsal root ganglia (DRG). Peak labeling occurred in T13 and L1 DRGs. Two distinct peaks were observed in cross-sectional areas of the labeled soma, and the mean cross-sectional area was 717.1 ± 27.7 μm2. Immunofluorescence staining for transient receptor potential cation channel subfamily V member 1 (TRPV1) separated DiI-positive neurons into three subpopulations: small TRPV1-negative, small TRPV1-positive, and large TRPV1-negative. Furthermore, the injection of RTX into PrAT reduced labeled cells by 36.7% where TRPV1-positive cells were the main target of RTX denervation. These novel findings provide a structural basis for future TRPV1-dependent and TRPV1-independent studies on the sensory innervation of PrAT, which may be of interest for future therapeutic obesity treatment and intervention.
Highlights
Obesity has become a global epidemic and has contributed to the increasing prevalence of cardiovascular diseases (CVD) (Hu et al, 2001; Coutinho et al, 2011; Foster et al, 2011)
Perirenal adipose tissue (PrAT) is a fat pad in retroperitoneal space, DiI tracing results show that sensory neurons that innervate rat PrAT reside in thoracolumbar dorsal root ganglia (DRG) only
This study identified the spinal DRGs involved in the primary afferent innervation of rat PrAT
Summary
Obesity has become a global epidemic and has contributed to the increasing prevalence of cardiovascular diseases (CVD) (Hu et al, 2001; Coutinho et al, 2011; Foster et al, 2011). Perirenal adipose tissue (PrAT), a fat pad located in the retroperitoneal space (Standring, 2005), has been. Sensory Innervation of Perirenal Fat recognized as a visceral adipose tissue that mediates CVD pathogenesis (Li et al, 2016; Ma et al, 2016; Zhao et al, 2016). Previous neural tracing studies have located sensory neurons projected into retroperitoneal viscera, such as the ureter and colon, in both thoracolumbar and sacral DRGs indicating dual sensory innervation (Robinson et al, 2004; Herweijer et al, 2014). We question whether dual sensory innervation exists in PrAT
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