Distribution characteristics and drug resistance of haemophilus influenzae at a hospital during 2016-2017

Abstract

Objective To provide some references for clinically and rationally using antibiotics retrospectively analyzing the distribution characteristics and of haemophilus influenzae and antimicrobial susceptibility results at the department of pediatrics of our hospital from January, 2016 to December, 2017. Methods Chocolate agar plates containing V and X factors were used to isolate haemophilus influenzae. VITEK-2 compact was applied to identify the species. Antimicrobial susceptibility was tested by Kirby-Bauermethod. Beta lactamases were detected with nitrocefin disk. Results A total of 156 strains of haemophilus influenzae were isolated. In the second quarter of each year, the detection rate was the highest, especially in children under 1 year old. The combination with streptococcus pneumoniae was common. The antimicrobial susceptibility results showed that the resistant rates of haemophilus influenzae to Smz-Tmp, CEC, CXM, and AZM were 75.9%, 81.3%, 41.1%, and 30.4%. And the susceptibility rates of haemophilus influenzae to CRO, LVX, and AMC were all over 95.0%.The positive rate of β-lactamses was 66.7%. No BLNAR strain was detected. Conclusions Haemophilus influenzae infection rate is high in infants under 1 year old, and seasonally changes. The drug resistant mechanism is complicated; and high positive rate of β-lactamses still is the important mechanism. In clinical practice, antibiotics should be chosen according to antimicrobial susceptibility results. Key words: Haemophilus influenzae; Infantile infection; β-lactamses; Antimicrobial susceptibility