Distribution and morphology of the ampullary organs of the salmontail catfish,Arius graeffei

Abstract

Whole body staining of Arius graeffei revealed that ampullary pores cover the body with their highest densities occurring on the head and lowest densities on the mid-ventral surface. Each ampullary organ consists of a long canal (0.2-1.75 mm) passing perpendicular to the basement membrane, through the epidermis into underlying dermal connective tissues, curving thereafter to run roughly parallel to the epidermis. Histochemical staining techniques (Alcian blue and Lillie's allochrome) indicate that the canals contain a neutral to acidic glycoprotein-based mucopolysaccharide gel that varies in composition along the length of the canal. Collagen fibers, arranged in a sheath, surround a layer of squamous epithelium that lines each ampullary canal. At the proximal end of the canal, squamous cells are replaced by cuboidal epithelial cells that protrude into the lumen, thus constricting the lumen to form a small pore into the ampulla. The ampulla is lined with receptor and supportive cells. The numerous (60-120) pear-shaped receptor cells bear microvilli on their luminal surface. Two forms of receptor cells exist in each ampullary organ: basal and equatorial receptor cells. Each receptor cell is connected to an unmyelinated nerve. Each receptor cell is surrounded by supportive cells on all but the apex. Tight junctions and underlying desmosomes occur between adjacent receptor and supportive cells. This form of ampullary organ has not previously been described for teleosts. J. Morphol. 239:97-105, 1999. © 1999 Wiley-Liss, Inc.