Abstract
Pollen isozyme systems of 19 cultivars of Diospyros kaki L., and 1 and 2 clones of D. lotus L. and D. virginiana L., respectively, were studied by starch gel electrophoresis in an attempt to discriminate between cultivars and species. Eight enzymes gave 4–20 different banding patterns, of which glucose phosphate isomerase (GPI) and phosphoglucomutase (PGM), both having a variety of well-resolved banding patterns, were thought to be the most useful enzymes for discriminating between the cultivars studied. Thirteen out of 19 cultivars were distinguished by either GPI or PGM. By using the two isozyme systems together, all cultivars were uniquely discriminated. D. lotus and D. virginiana showed unique banding patterns not observed with D. kaki. Based on similarity index values calculated from 9 isozyme systems, some conclusions could be drawn on the possible relationships among the cultivars and species examined.
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