Abstract
Viral metagenomic analysis identified a new parvovirus genome in the intestinal contents of wild shrews in Zambia. Related viruses were detected in spleen tissues from wild shrews and nonhuman primates. Phylogenetic analyses showed that these viruses are related to human bufaviruses, highlighting the presence and genetic diversity of bufaviruses in wildlife.
Highlights
Viral metagenomic analysis identified a new parvovirus genome in the intestinal contents of wild shrews in Zambia
We found that the amino acid sequence identity of VP1 between Mpulungu BuV (MpBuV) and human BuV is 52.3%, whereas that of VP2 is 51.4%
The nearly complete genome sequence of a new parvovirus, MpBuV, was obtained from a wild shrew in this study. blastp searches indicated that each MpBuV open reading frame shared the highest amino acid identity with other known BuVs
Summary
Viral metagenomic analysis identified a new parvovirus genome in the intestinal contents of wild shrews in Zambia. We previously described the enteric virome of wild shrews of the Crocidura genus sampled at Mpulungu, Zambia, in 2012 [6]. Ncbi.nlm.nih.gov) searches showed that the MpBuV NS1, VP1, and VP2 proteins were closely related to those of human BuVs and the WUHARV parvovirus (E-value = 0.0). MpBuV clustered with human BuVs and WUHARV parvovirus (online Technical Appendix Figure). PCR was performed by using Tks Gflex DNA polymerase (TAKARA BIO, Otsu, Japan), forward primer MpBuV-F1 (position 2739–2763 in MpBuV genome, 5′-GAAGTGGTGTTGGTCATTCTACTGG-3′) and reverse primer MpBuV-R1
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