Abstract
The concentration of fetal calf serum (FCS) and the culture period were the crucial culture conditions in measuring B cell growth factor (BCGF) and IL-2 activity in vitro. Higher concentrations of FCS (10–15%) significantly inhibited BCGF activity; whereas, lower concentrations of FCS (<2.5%) were not sufficient for the response to IL-2. Kinetic experiments showed that the culture period for BCGF should be shorter than that for IL-2, while BCGF in combination with IL-2 induced a synergistic proliferation of B cells in a longer culture period. Adding BCGF after 48 h of SAC stimulation reduced the reaction. Hence, the conventional method using preactivated B cells does not measure BCGF but mostly IL-2. Furthermore, minute amounts of BCGF activity can be more sensitively determined by co-culturing with fixed amounts of IL-2.
Published Version
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