Abstract
The last decade has seen an enormous increase in interest to structural studies in biological systems using pulsed EPR techniques, in particular, to the distance measurements between pairs of paramagnetic centers using pulsed ELDOR and a whole arsenal of other techniques. Most notable applications are to the distance measurements between spin labels in double-labeled proteins and between electron transfer intermediates in photosynthetic reaction centers. In this chapter we will describe the theoretical and practical aspects of pulsed ELDOR and a related technique, 2+1, and demonstrate their application to measurements of distances between electron transfer cofactors in plant Photosystem II (PS II) and other photosynthetic reaction centers (RC). Since much of the distance information in photosynthetic RCs was obtained by pulsed ELDOR and 2+1, these are the main techniques we discuss. However, the results obtained by other pulsed EPR methods, the selective hole burning and out-of-phase electron-spin echo envelope modulation, will also be mentioned.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
