Abstract
Soil-borne cereal mosaic virus(SBCMV), the causative agent of wheat mosaic, is aFuroviruschallenging wheat production all over Europe. Differently from bread wheat, durum wheat shows greater susceptibility and stronger yield penalties, so identification and genetic characterization of resistance sources are major targets for durum genetics and breeding. TheSbm1locus providing high level of resistance to SBCMV was mapped in bread wheat to the 5DL chromosome arm (Bass in Genome 49:1140-1148, 2006). This excluded the direct use ofSbm1for durum wheat improvement. Only one major QTL has been mapped in durum wheat, namelyQSbm.ubo-2B,on the 2BS chromosome region coincident withSbm2, already known in bread wheat as reported (Bayles in HGCA Project Report, 2007). Therefore,QSbm.ubo-2B = Sbm2is considered a pillar for growing durum in SBCMV-affected areas. Herein, we report the fine mapping ofSbm2based on bi-parental mapping and GWAS,using the Infinium 90KSNP array and high-throughput KASP®. Fine mapping pointed out a critical haploblock of 3.2Mb defined byconcatenated SNPs successfully converted to high-throughput KASP®markers coded asKUBO. The combination ofKUBO-27,wPt-2106-ASO/HRM,KUBO-29,andKUBO-1allows unequivocal tracing of theSbm2-resistant haplotype. The interval harbors 52 high- and 41 low-confidence genes, encoding 17 cytochrome p450, three receptor kinases, two defensins, and threeNBS-LRRgenes. These results pave the way forSbm2positional cloning.Importantly, the development ofSbm2 haplotype tagging KASP®provides a valuable case study for improving efficacyof the European variety testing system and, ultimately, the decision-making process related to varietal characterization and choice.
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