Abstract
As one of the pivotal signal molecules, hydrogen peroxide (H2O2) has been demonstrated to play important roles in many physiological processes of plants. Continuous monitoring of H2O2 in vivo could help understand its regulation mechanism more clearly. In this study, a disposable electrochemical microsensor for H2O2 was developed. This microsensor consists of three parts: low-cost stainless-steel wire with a diameter of 0.1 mm modified by gold nanoparticles (disposable working electrode), an untreated platinum wire with a diameter of 0.1 mm (counter electrode), and an Ag/AgCl wire with a diameter of 0.1 mm (reference electrode), respectively. The microsensor could detect H2O2 in levels from 10 to 1000 µM and exhibited excellent selectivity. On this basis, the dynamic change in H2O2 in the vein of tomato leaf under high salinity was continuously monitored in vivo. The results showed that the production of H2O2 could be induced by high salinity within two hours. This study suggests that the disposable electrochemical microsensor not only suits continuously detecting H2O2 in microscopic plant tissue in vivo but also reduces the damage to plants. Overall, our strategy will help to pave the foundation for further investigation of the generation, transportation, and elimination mechanism of H2O2 in plants.
Highlights
Hydrogen peroxide (H2O2), a simple molecular compound discovered by Louis Jacques Denard a hundred years ago, is one of the crucial members of the reactive oxygen species (ROS) [1,2]
This study suggested that the disposable electrochemical microsensor is suitable for continuously detecting H2O2 in microscopic plant tissue in vivo but can reduce the damage to plants, which will help pave the foundation for further investigation of the generation, transportation, and elimination mechanisms of H2O2 in plants
The typical amperometric (i-t) curves of the Au modified bare SS electrode (Au/SS) wire-based electrochemical microsensor were recorded by the successive addition of H2O2 with different concentrations into 0.2 M phosphate-buffered solution (PBS) solution at an applied potential of −0.6 V (Figure 4)
Summary
Hydrogen peroxide (H2O2), a simple molecular compound discovered by Louis Jacques Denard a hundred years ago, is one of the crucial members of the reactive oxygen species (ROS) [1,2]. Studies about the roles of H2O2 in the signal transduction, especially in crosstalk with the plant hormones in the stresses have made great progress [1,11], direct experimental evidence is lacking regarding the sites, initiation time, and transportation of H2O2 Obtaining this evidence is limited on how to determine the concentration distribution and dynamic change in H2O2 in plants. This study suggested that the disposable electrochemical microsensor is suitable for continuously detecting H2O2 in microscopic plant tissue in vivo but can reduce the damage to plants, which will help pave the foundation for further investigation of the generation, transportation, and elimination mechanisms of H2O2 in plants
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