Abstract
Thin-layer chromatography has several advantages in comparison to column chromatography, e.g., simultaneous separation of several samples, easy realization of two-dimensional separation, direct observation of both the chromatographic development and the separation using colored compounds, sensitive and easy detection of the spots with specific spray reagents. The entire procedure, i.e., loading, development, and evaluation are relatively simple. An additional feature is the optimization possibilities of mobile phase flow velocity, based on the complexity of driving force where capillarity results in a concave flow profile that may be counterbalanced by convex profile of laminar flow. Displacement thin-layer chromatography has the advantages of planar arrangement of the stationary phase, and opens new possibilities for analytical separations.This paper outlines basic features of planar displacement chromatography, including differences from elution chro-matography, and that of column displacement chromatography, the possibility of using spacer displacement chromatography. Parameters limiting displacement chromatography, and their optimization to achieve better separation are discussed.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
