Abstract

Antibiotics are widely used to prevent diseases and promote growth in food – producing animals. Their usage may result in the presence of antibiotics in food and environmental samples. Antibiotics analysis in complex samples, such as food and environment samples, require sample pretreatment. Application of activated carbon, C18 and florisil and the influence of the amount of applied sorbents on their effectiveness in dispersive solid phase extraction (dSPE) for chloramphenicol and tetracycline analysis was examined. Activated carbon showed the lowest efficiency in the extraction of antibiotics (29% when analyzing samples containing chloramphenicol when 0.05 g of sorbent was added). When analyzing samples containing chloramphenicol, florisil showed an equal efficiency for all three sorbent amounts (92%), so extraction efficiency when using florisil does not depend on the mass of applied sorbent. Octadecyl silica (C18) shows high efficiency when analyzing chloramphenicol and tetracycline (96% in samples containing chloramphenicol, and 102% in samples containing tetracycline), so it can be applied in chloramphenicol and tetracycline analysis.

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