Abstract
Introduction: As the prevalence of obesity rises, so does the need for reliable Body Composition (BC) methods. Many methods of BC analysis exist, however validity between methods has been questioned. Uncertainty exists within the scientific literature about which laboratory method and field measurement is applicable for the overweight population to estimate Percent Body Fat (% BF). There is scarce research comparing Hydrostatic Weighing (HW) and Dual Energy X-Ray Absorptiometry (DXA) with field methods in overweight individuals. The purpose of this study was to compare HW and DXA with Multi-Frequency (MF-BIA) and a Single-Frequency (SFBIA) Bioelectrical Impedance (BIA) device. Methods: Twenty-five overweight and obese adults (BMI 30.1 ± 4.1 kg/ m2, 13 males, 12 females, ages 24.6 ± 4.7y) were randomly assessed by vertical SF-BIA, and supine MF-BIA against DXA and lastly HW. All participants followed standardized pre-testing guidelines for BC assessment. Agreement was assessed using a 2 (group) x 4 (method) RMANOVA and post-hoc t-tests were applied as appropriate. All methods of body composition were used and compared to DXA and HW. You can submit your Manuscripts at: https://symbiosisonlinepublishing.com/submitManuscript.php
Highlights
As the prevalence of obesity rises, so does the need for reliable Body Composition (BC) methods
% BF values were higher using the MF-Bioelectrical Impedance (BIA) (30.9 ± 58.8 %) compared to the Skin Fold Measurement (SF)-BIA (26.2 ± 8.0%) (p < 0.001). %BF values were significantly correlated between SF-BIA and Hydrostatic Weighing (HW) (p < 0.05, r = 0.902) as well as between MF-BIA and Dual Energy X-Ray Absorptiometry (DXA) (p < 0.05, r = 0.709)
The field method SF-BIA was found to correlate with the laboratory method HW
Summary
As the prevalence of obesity rises, so does the need for reliable Body Composition (BC) methods. HW is limited by several inherent sources of error, these largely stem from variability in residual airway volume [5], and the dependence of this method on a number of static reference values, drawn from limited animal and cadaver research performed in the 1940’s and 50’s [9,10,11] These include static assumptions regarding the densities of Fat Mass (FM) (0.9007 g/ cm3), and Fat-Free Mass (FFM) (1.100 g/ cm3), as well as the water content of FFM (73.2 %), which are often uniformly applied to all subjects irrespective of age, ethnicity, or body composition [12].
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