Disentangling feedforward versus feedback processing in numerosity representation

Abstract

Numerosity is a fundamental aspect of the external environment, needed to guide our behavior in an effective manner. Previous studies show that numerosity processing involves at least two temporal stages (~100 and ~150 msec after stimulus onset) in early visual cortex. One possibility is that the two stages reflect an initial feedforward processing followed by feedback signals from higher-order cortical areas that underlie segmentation of visual inputs into perceptual units that define numerosity. Alternatively, multiple stages of feedforward processing might progressively refine the input leading to the segmented representation. Here, we distinguish these two hypotheses by exploiting the connectedness illusion (i.e., the systematic underestimation of pairwise-connected dots), backward masking (to suppress feedback signals), and serial dependence (i.e., a perceptual bias making a stimulus appear to be more similar to its preceding one). Our results show that a connected dot array biases the numerosity representation of the subsequent dot array based on its illusory perception, irrespective of whether it is visible or suppressed by masking. These findings demonstrate that feedback processing is not strictly necessary for the perceptual segmentation that gives rise to perceived numerosity, and instead suggest that different stages of feedforward activity presumably carrying low and high spatial frequency information are sufficient to create a numerosity representation in early visual areas.